Category Archives: 3-D Printing

3D-bioprinted human skin can replace animal testing, potentially be used in burns

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José Luis Jorcano at Universidad Carlos III de Madrid has developed a 3D bioprinter capable of replicating the structure of skin. The human-like  skin that is produced  includes an epidermal layer that protects against the environment, and a collagen-producing dermis that provides elasticity and strength.

The bioink material  contains human plasma, and  primary human fibroblasts and keratinocytes obtained from biopsies.

Currently, 100 cm2 of the printed skin  is able to be produced in 35 minutes.

ApplySci’s 6th  Digital Health + NeuroTech Silicon Valley  –  February 7-8 2017 @ Stanford   |   Featuring:   Vinod Khosla – Tom Insel – Zhenan Bao – Phillip Alvelda – Nathan Intrator – John Rogers – Roozbeh Ghaffari –Tarun Wadhwa – Eythor Bender – Unity Stoakes – Mounir Zok – Sky Christopherson – Marcus Weldon – Krishna Shenoy – Karl Deisseroth – Shahin Farshchi – Casper de Clercq – Mary Lou Jepsen – Vivek Wadhwa – Dirk Schapeler – Miguel Nicolelis

3D printed renal architecture

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Harvard’s Jennifer Lewis and Roche’s  Annie Moisan have used 3D printing to fabricate a small but critical subunit of a kidney.  The renal architecture contains living epithelial cells.

Earlier bioprinting approaches were adapted to form thick tissues.  A 3D-printed silicone gasket was used to cast an engineered extracellular matrix as a base layer. “Fugitive ink” was printed in a shape similar to that of renal proximal tubules, and encapsulated with another layer of extracellular matrix.

The in vitro model functions like living kidney tissue, representing a significant advance from traditional 2D cell culture.  The result could be an implant or assistive device, and/or more effective clinical trials.

Click to view Wyss Institute video.


ApplySci’s 6th   Wearable Tech + Digital Health + NeuroTech Silicon Valley  –  February 7-8 2017 @ Stanford   |   Featuring:   Vinod Khosla – Tom Insel – Zhenan Bao – Phillip Alvelda – Nathan Intrator – John Rogers – Mary Lou Jepsen – Vivek Wadhwa – Miguel Nicolelis – Roozbeh Ghaffari –Tarun Wadhwa – Eythor Bender – Unity Stoakes – Mounir Zok – Krishna Shenoy – Karl Deisseroth

3D printed gel model replicates brain folding mechanism

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L. Mahadevan and Harvard colleagues have  used 3D printing to replicate a folding human brain.  The goal is to understand how brain folds are related to disease. While many molecular processes  determine cellular events, the study shows that what ultimately causes the brain to fold is a mechanical instability associated with buckling.
A 3D  gel model of a smooth fetal brain was created based on MRI images. To mimic cortical expansion, the gel brain was immersed in a solvent that is absorbed by the outer layer, causing it to swell relative to the deeper regions. The resulting compression led to the formation of folds similar in size and shape to real brains.
In humans, folding begins in fetal brains at the 20th week of gestation,  and is completed at a year and a half. The number, size, shape and position of neuronal cells during brain growth lead to the expansion of the cortex (gray matter), relative to the underlying white matter. The scientists said that this puts the cortex under compression, leading to a mechanical instability that causes it to crease locally. They believe that if a part of the brain does not grow properly, or if the global geometry is disrupted, the major folds may not be in the right place, which may cause dysfunction.

Wearable Tech + Digital health San Francisco – April 5, 2016 @ the Mission Bay Conference Center

NeuroTech San Francisco – April 6, 2016 @ the Misson Bay Conference Center

Wearable Tech + Digital Health NYC – June 7, 2016 @ the New York Academy of Sciences

NeuroTech San Francisco – June 8, 2016 @ the New York Academy of Sciences

3D printed model for brain aneurysm surgery planning

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Stratasys and the Jacobs Institute have used 3D printing for brain surgery planning in an effort to reduce risk. Anatomical models of a patient’s entire brain vessel anatomy were 3D printed before she underwent an aneurysm procedure.

The replica, built of a polymer that mimics human tissue, allowing the surgeons to plan their approach and practice the operation, was based on CT scans.

In this case. the accurate model enabled surgeons to fine-tune the procedure.  “While we were doing that mock procedure, we realized that we had to change some of the tools we wanted to use, given her anatomy,” said  Adnan Siddiqui, Jacobs’ Chief Medical Officer.

WEARABLE TECH + DIGITAL HEALTH SAN FRANCISCO – APRIL 5, 2016 @ THE MISSION BAY CONFERENCE CENTER

NEUROTECH SAN FRANCISCO – APRIL 6, 2016 @ THE MISSION BAY CONFERENCE CENTER

Toward a 3D printed heart

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Carnegie Mellon‘s Adam Feinberg is developing 3D printing techniques that could in the future be used to repair the heart.  This work is aimed at alternative solutions for the 4,000 Americans currently waiting to receive a heart transplant.

Feinberg described his progress:  “We’ve been able to take MRI images of coronary arteries and 3-D images of embryonic hearts and 3-D bioprint them with unprecedented resolution and quality out of very soft materials like collagens, alginates and fibrins.”

The next step is to incorporate real heart cells into these 3-D printed tissue structures, providing a scaffold to help form contractile muscle.

Click to view Carnegie Mellon video.

WEARABLE TECH + DIGITAL HEALTH SAN FRANCISCO – APRIL 5, 2016 @ THE MISSION BAY CONFERENCE CENTER

NEUROTECH SAN FRANCISCO – APRIL 6, 2016 @ THE MISSION BAY CONFERENCE CENTER

Faster, personalized, 3D printed heart models for surgery planning

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MIT and Boston Children’s Hospital researchers are converting heart MRI scans into 3D printed physical models,  for surgical planning,  in 3-4 hours.  Previously, the process took 10 hours. The project, which limits human input to increase accuracy, is led by Professor Polina Golland.  Physicist Medhi Moghari enhanced the precision of the MRI, decreasing the dependence on generic models, and enabling the the team to create the algorithm and print the model in the shorter time frame.

The algorithm examines patches of unsegmented cross sections and looks for similar features in the nearest segmented cross sections. Golland believes that its performance might be improved if it also examined patches that ran obliquely across several cross sections, which will be the next phase of research.

Cheap, accurate, 3D printed stethoscope

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Dr. Tarek Loubani has created a 3D printed stethoscope that can be made for $2.50 – $5.00.  Stethoscopes usually cost $150 and are often not available in poor regions.

Through his Glia Project, Dr. Loubani aims to provide cheap, accurate medical supplies, including stethoscopes, electrocardiograms, and pulse oximeters,  to places in need.

“This is simple, cheap and it’s enough for us here,” said Dr. Ayman Sahbani, head of the emergency department at Gaza’s Shifa Hospital, who tested the Glia stethoscope. “Now we can make a stethoscope available for each doctor.”

Cancer patient receives 3D printed rib cage

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For the first time, a chest wall sarcoma patient has received a  fully customized 3d printed sternum and rib cage portion, created using high resolution CT data.

This part of the chest is difficult to recreate with traditional prosthetics.  Thoracic surgeons typically use flat and plate implants for the chest, which can loosen over time and increase complications.  Rapidly prototyped 3D printed ribs may become the future standard.

View CSIRO video here.

3D printed airway splints restore breathing

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At the University of Michigan, three children under 2 with tracheobronchomalacia had 3D printed devices implanted to open their airways and restore their breathing.

Professors Glenn Green and Scott Hollister were able to create and implant customized tracheal splints for each patient. The device was created directly from CT scans of their tracheas, integrating an image-based computer model with laser-based 3D printing to produce the splint.

The splint was sewn around the patient’s airways to expand the trachea and bronchus and give it a skeleton to aid proper growth. It is designed to be reabsorbed by the body over time. The growth of the airways were followed with CT and MRI scans, and it was shown to allow airway growth for all three patients.

The findings suggest that early treatment of tracheobronchomalacia may prevent complications of conventional treatment such as a tracheostomy, prolonged hospitalization, mechanical ventilation, cardiac and respiratory arrest, food malabsorption and discomfort. None of the devices implanted in this study have caused complications.

The bioresorable splints enabled the patients to come off of ventilators and ended their need for paralytics, narcotics and sedation.  Researchers noted improvements in multiple organ systems.  The patients were also relieved of immunodeficiency-causing proteins that prevented them from absorbing food so that they no longer needed intravenous therapy.

WEARABLE TECH + DIGITAL HEALTH NYC 2015 – JUNE 30 @ NEW YORK ACADEMY OF SCIENCES.  REGISTER HERE.

3-D printed organs interlaced with blood vessels

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MIT Technology Review

Harvard professor Jennifer Lewis has created a patch of tissue containing skin cells and biological structural material interwoven with blood-vessel-like structures using a 3-D printer and “disappearing” ink.

Lewis’s team created hollow, tube-like structures within a mesh of printed cells using an “ink” that liquefies as it cools. The tissue is built by the 3-D printer in layers. A gelatin-based ink acts as extracellular matrix—the structural mix of proteins and other biological molecules that surrounds cells in the body. Two other inks contained the gelatin material and either mouse or human skin cells. All these inks are viscous enough to maintain their structure after being laid down by the printer.

A third ink with counterintuitive behavior helped them create the hollow tubes. This ink has a Jell-O-like consistency at room temperature, but when cooled it liquefies. The team printed tracks of this ink amongst the others. After chilling the patch of printed tissue, the researchers applied a light vacuum to remove the special ink, leaving behind empty channels within the structure. Then cells that normally line blood vessels in the body can be infused into the channels.

The smallest channels printed were about 75 micrometers in diameter, which is much larger than the tiny capillaries that exchange nutrients and waste throughout the body. The hope is that the 3-D printing method will set the overall architecture of blood vessels within artificial tissue and then smaller blood vessels will develop along with the rest of the tissue.